E like me ka hoʻolālā ʻana o nā mea hoʻolālā kūlanakauhale i ka holo ʻana o ke kaʻa ma nā kikowaena o ke kūlanakauhale, hoʻokele pono nā cell i ka neʻe ʻana o ka molekala ma ko lākou mau palena nuklea. Ke hana nei ma ke ʻano he kiaʻi puka microscopic, nā nuclear pore complexes (NPCs) i hoʻokomo ʻia i loko o ka membrane nuklea e mālama pono i kēia kālepa molekala. ʻO ka hana hoʻoheheʻe honua mai Texas A&M Health e hōʻike ana i ka koho maʻalahi o kēia ʻōnaehana, hiki ke hāʻawi i nā hiʻohiʻona hou i nā maʻi neurodegenerative a me ka ulu ʻana o ka maʻi kanesa.
Ka Huli Kipi o nā Ala Molecular
Ua hoʻomaka ka hui noiʻi a Kauka Siegfried Musser ma Texas A&M College of Medicine i nā noiʻi ʻana i ka holo wikiwiki ʻana o nā molekala ma o ka pale pālua-membrane o ka nucleus. ʻO kā lākou palapala hoʻolaha ʻāina ʻo Nature e hōʻike ana i nā ʻike kipi i hiki i ka ʻenehana MINFLUX - kahi ʻano kiʻi kiʻi kiʻi kiʻi hiki ke hopu i nā neʻe molekala 3D e kū nei i loko o nā milliseconds ma nā unahi ma kahi o 100,000 mau manawa ʻoi aku ka ʻoi aku o ka laulā o ka lauoho kanaka. Kūlike ʻole i nā manaʻo mua e pili ana i nā ala kaʻawale, hōʻike kā lākou noiʻi ʻana i nā kaʻina hoʻokomo nuklea a me nā kaʻina hoʻokuʻu aku e kaʻana like i nā ala i loko o ka hale NPC.
ʻO nā mea hoʻohālike e kū nei i kēia manawa
Hōʻike ka ʻike a ka hui i nā ʻano kaʻahele i manaʻo ʻole ʻia: hoʻokele bidirectionally nā molekala ma nā ala paʻa, e hoʻololi ana kekahi i kekahi ma mua o ka hahai ʻana i nā ala i hoʻolaʻa ʻia. ʻO ka mea kupaianaha, ke hoʻomau nei kēia mau ʻāpana kokoke i nā paia o ke kahawai, e waiho ana i ka ʻāpana waena, ʻoiai ke lohi nei ko lākou holomua - ma kahi o 1,000 mau manawa ʻoi aku ka lohi ma mua o ka neʻe ʻana ʻole - ma muli o nā ʻupena protein obstructive e hana ana i kahi ʻano syrupy.
Ua wehewehe ʻo Musser i kēia ma ke ʻano "ʻo ke ʻano paʻakikī paʻakikī loa e hiki ke noʻonoʻo ʻia - kahe ʻelua ala ma nā ala haiki." Ua ʻae ʻo ia, "Ke hōʻike nei kā mākou ʻike i kahi hui like ʻole o nā mea hiki, e hōʻike ana i ka paʻakikī ʻoi aku ka paʻakikī ma mua o kā mākou mau kuhiakau mua i manaʻo ʻia."
ʻO ka maikaʻi me nā pilikia
ʻO ka mea kupaianaha, hōʻike nā ʻōnaehana kaʻa NPC i ka maikaʻi maikaʻi loa ʻoiai kēia mau kaohi. Manaʻo ʻo Musser, "ʻO ka nui maoli o nā NPC hiki ke pale i ka hana overcapacity, me ka hōʻemi ʻana i ka hoʻokūkū hoʻokūkū a me nā pilikia paʻa." ʻIke ʻia kēia hiʻohiʻona hoʻolālā i ka pale ʻana i ka molecular gridlock, Eia'i ka mana i kākau hou ʻia me nā syntax like ʻole, ka hoʻolālā, a me nā ʻāpana paukū ʻoiai e mālama ana i ke ʻano kumu:
Holo ka Molecular Traffic i kahi alahele: Hōʻike nā NPC i nā ala huna
Ma kahi o ka hele pololei ʻana ma o ka NPC's ke koʻikoʻi waena, ʻike ʻia nā molekala e hoʻokele i kekahi o nā ala kaʻa kūikawā ʻewalu, paʻa kēlā me kēia i kahi ʻano like me ka ʻōlelo ma ka pore.'s apo puka waho. Hōʻike kēia hoʻonohonoho spatial i kahi ʻano hana hoʻolālā i lalo e kōkua i ka hoʻoponopono ʻana i ke kahe molekala.
Wahi a Musser,“ʻOiai ua ʻike ʻia nā pores nuklea hū he a'plug waena,'he mea pohihihi kona haku mele ana. I loko o nā pūnaewele kanaka, loaʻa kēia hiʻohiʻona'ua ʻike ʻia, akā hiki ke hoʻokaʻawale ʻia ka hana—a me ka pore'Hiki i ke kikowaena ke lilo i ala nui no ka mRNA.”
Nā pilina maʻi a me nā pilikia lapaʻau
Hoʻopilikia i ka NPC—he ʻīpuka kelepona koʻikoʻi—ua hoʻopaʻa ʻia i nā maʻi neurological koʻikoʻi, me ka ALS (Lou Gehrig's maʻi), Alzheimer's, a me Huntington's maʻi. Hoʻohui ʻia, pili ka hana trafficking NPC kiʻekiʻe i ka piʻi ʻana o ka maʻi kanesa. ʻOiai ʻo ka ʻimi ʻana i nā wahi pore kikoʻī hiki ke kōkua i ka wehe ʻana i nā blockages a i ʻole e hoʻolōʻihi i ka lawe ʻana i ka nui, ua ʻōlelo ʻo Musser i ka hoʻopili ʻana me ka hana NPC e lawe ana i nā pilikia, hāʻawi ʻia i kāna kuleana nui i ke ola cell.
“Pono mākou e hoʻokaʻawale i waena o nā hemahema pili i ka halihali a me nā pilikia i pili i ka NPC's hui a wehe ʻana paha,”hoʻomaopopo ʻo ia.“ʻOiai e hāʻule ana ka nui o nā pilina maʻi i ka ʻāpana hope, aia nā ʻokoʻa—e like me ka c9orf72 gene mutations ma ALS, ka mea i hana i na aggregates e keakea kino ana i ka pore.”
Nā kuhikuhi i ka wā e hiki mai ana: ka palapala ʻana i nā ala ʻauke a me ke kiʻi kiʻi ola-cell
Musser a me Kauka Abhishek Sau, mai Texas A&M's Joint Microscopy Lab, e hoʻolālā e noiʻi i nā ʻano ukana like ʻole—e like me ribosomal subunits a me mRNA—hahai i nā ala kū hoʻokahi a i ʻole e hui pū ma nā ala like. ʻO kā lākou hana mau me nā hoa Kelemania (EMBL a me Abberior Instruments) hiki ke hoʻololi i ka MINFLUX no ke kiʻi ʻana i ka manawa maoli i nā cell ola, e hāʻawi ana i nā manaʻo like ʻole o ka dynamics transport nuclear.
Kākoʻo ʻia e ke kālā NIH, hoʻoponopono hou kēia haʻawina i ko mākou ʻike ʻana i ka logistic cellular, e hōʻike ana i ka mālama ʻana o nā NPC i ka hoʻonohonoho ʻana i ka metropolis microscopic bustling o ka nucleus.
Ka manawa hoʻouna: Mar-25-2025
