E like me nā mea hoʻolālā kūlanakauhale e hoʻonohonoho pono ana i ke kahe o nā kaʻa ma nā kikowaena kūlanakauhale, hoʻomalu pono nā cell i ka neʻe ʻana o nā molekala ma waena o ko lākou mau palena nuklea. Ma ke ʻano he mau kiaʻi puka microscopic, mālama nā complexes pore nuklea (NPC) i hoʻokomo ʻia i loko o ka membrane nuklea i ka mana pololei ma luna o kēia kālepa molekala. Ke hōʻike nei ka hana groundbreaking mai Texas A&M Health i ke koho akamai o kēia ʻōnaehana, e hāʻawi ana i nā manaʻo hou i nā maʻi neurodegenerative a me ka ulu ʻana o ka maʻi kanesa.
Ka Hahai Kipi ʻana o nā Ala Molekala
Ua hoʻomaka ka hui noiʻi a Kauka Siegfried Musser ma ke Kulanui Lapaʻau ʻo Texas A&M i nā noiʻi i ka neʻe wikiwiki ʻana o nā molekala ma o ka pale membrane pālua o ka nucleus. Hōʻike kā lākou puke pai koʻikoʻi ʻo Nature i nā ʻike hou i hiki ke hana ʻia e ka ʻenehana MINFLUX - kahi ʻano hana kiʻi holomua i hiki ke hopu i nā neʻe molekala 3D e hana ʻia ana i loko o milliseconds ma nā unahi ma kahi o 100,000 mau manawa ʻoi aku ka maikaʻi ma mua o ka laulā o ka lauoho kanaka. Kūʻē i nā kuhi mua e pili ana i nā ala i hoʻokaʻawale ʻia, hōʻike kā lākou noiʻi e kaʻana like nā kaʻina hana lawe mai a me ka hoʻokuʻu aku i nā ala e pili ana i loko o ka ʻōnaehana NPC.
Hoʻokūkū nā ʻIke Kupaianaha i nā Hoʻohālike Kū i kēia manawa
Ua hōʻike nā nānā ʻana a ka hui i nā ʻano kaʻa i manaʻo ʻole ʻia: hoʻokele nā molekala ma ke ʻano bidirectional ma o nā kahawai i hoʻopaʻa ʻia, e neʻe ana a puni kekahi i kekahi ma mua o ka hahai ʻana i nā ala i hoʻolaʻa ʻia. ʻO ka mea kupaianaha, ua hoʻopili kēia mau ʻāpana ma kahi kokoke i nā paia o ke kahawai, e waiho ana i ka wahi waena hakahaka, ʻoiai e lohi nui ana ko lākou holomua - ma kahi o 1,000 mau manawa lohi ma mua o ka neʻe ʻole ʻia - ma muli o nā pūnaewele protein obstructive e hana ana i kahi ʻano syrupy.
Ua wehewehe ʻo Musser i kēia ʻo "ke kūlana kaʻa paʻakikī loa i hiki ke noʻonoʻo ʻia - ke kahe ʻana o ʻelua ala ma o nā ala haiki." Ua ʻae ʻo ia, "Hōʻike kā mākou mau ʻike i kahi hui pū ʻana o nā mea hiki ke hana ʻia, e hōʻike ana i ka paʻakikī nui aʻe ma mua o kā mākou mau kuhiakau mua i manaʻo ʻia."
Ka Pono ʻOiai nā Palekana
ʻO ka mea kupanaha, hōʻike nā ʻōnaehana halihali NPC i ka pono kupaianaha me ka nānā ʻole i kēia mau palena. Ua kuhi ʻo Musser, "ʻO ka nui kūlohelohe o nā NPC e pale aku i ka hana overcapacity, e hōʻemi pono ana i ka hoʻopilikia hoʻokūkū a me nā pilikia blockaging." ʻIke ʻia kēia hiʻohiʻona hoʻolālā kūlohelohe e pale aku i ka gridlock molecular, Eia'sa mana i kākau hou ʻia me nā ʻano syntax like ʻole, ka hoʻonohonoho ʻana, a me nā haki paukū ʻoiai e mālama ana i ka manaʻo kumu:
Lawe ke Kaʻa Molecular i kahi Detour: Hōʻike nā NPC i nā Ala Huna
Ma kahi o ka hele pololei ʻana ma o ka NPC'Ma ke kikowaena o ke axis, me he mea lā e holo ana nā molekala ma o kekahi o nā kahawai halihali kūikawā ʻewalu, i hoʻopaʻa ʻia kēlā me kēia i kahi ʻano like me ka ʻōlelo ma ka pore.'ke apo o waho. Hōʻike kēia hoʻonohonoho ākea i kahi ʻano hana hoʻolālā kumu e kōkua ai i ka hoʻoponopono ʻana i ke kahe molekala.
Ua wehewehe ʻo Musser,"ʻOiai ua ʻike ʻia nā pores nuklea hū he'plug waena,'he mea pohihihi kona ʻano kikoʻī. I loko o nā pūnaewele kanaka, ua loaʻa kēia hiʻohiʻona'ʻaʻole i ʻike ʻia, akā hiki ke kūpono ka hoʻokaʻawale ʻana o ka hana—a me ka pore'hiki i ke kikowaena s ke lilo i ala hoʻokuʻu aku nui no ka mRNA."
Nā Pilina Maʻi a me nā Pilikia Lapaʻau
Ka hana ʻino ma ka NPC—he puka komo kelepona koʻikoʻi—ua pili pū ʻia me nā maʻi neurological koʻikoʻi, me ALS (Lou Gehrig'ka maʻi (s), Alzheimer's, a me Huntington'ka maʻi. Eia kekahi, ua pili ka hana hoʻonui ʻia o ka hana kālepa NPC i ka holomua o ka maʻi kanesa. ʻOiai ʻo ka hoʻopaʻa ʻana i nā wahi pore kikoʻī e hiki ke kōkua i ka wehe ʻana i nā poloka a i ʻole ka lohi o ka lawe nui ʻana, ua ʻōlelo ʻo Musser ʻo ka hoʻopilikia ʻana i ka hana NPC e lawe mai i nā pilikia, no ka mea he kuleana koʻikoʻi ia i ke ola ʻana o nā cell.
"Pono mākou e hoʻokaʻawale ma waena o nā hemahema e pili ana i ka halihali a me nā pilikia e pili ana i ka NPC'ka ʻākoakoa ʻana a i ʻole ka wehe ʻana,"hoʻomaopopo ʻo ia."ʻOiai he nui nā pilina maʻi e hāʻule ana i loko o ka māhele hope, aia nā ʻokoʻa—e like me nā hoʻololi ʻana o ka gene c9orf72 ma ALS, ka mea e hana i nā hōʻuluʻulu e ālai kino i ka pore."
Nā Kuhikuhi no ka Wā E Hiki Mai Ana: Ke Palapala ʻĀina ʻana i nā Alanui Ukana a me ke Kiʻi ʻana i nā Kelepona Ola
ʻO Musser a me kāna hoa hana ʻo Kauka Abhishek Sau, mai Texas A&M'ʻO ka Joint Microscopy Lab, hoʻolālā e noiʻi inā he ʻano ukana like ʻole—e like me nā subunits ribosomal a me ka mRNA—e hahai i nā ala kūikawā a i ʻole e hui pū ma nā ala i kaʻana like ʻia. ʻO kā lākou hana mau me nā hoa Kelemania (EMBL a me Abberior Instruments) hiki ke hoʻololi iā MINFLUX no ke kiʻi ʻana i ka manawa maoli i loko o nā cell ola, e hāʻawi ana i nā hiʻohiʻona i ʻike ʻole ʻia o ka dinamika halihali nuklea.
Me ke kākoʻo ʻia e ke kālā NIH, hoʻololi hou kēia haʻawina i ko mākou ʻike i ka logistics cellular, e hōʻike ana pehea e mālama ai nā NPC i ke kauoha i loko o ke kūlanakauhale microscopic piha o ka nucleus.
Ka manawa hoʻouna: Malaki-25-2025
